Additional Cell Culture Reagents and Supplements
STEMCELL Technologies CloneR™2, Size: 25 mL
Defined supplement for improving survival of human ES and iPS cells in single-cell workflows
Cell Biologics 0.1 % Gelatin-Based Coating Solution- Ready to use, 100ml
0.1 % Gelatin-Based Coating Solution is designed to coat cell culture flasks or dishes. Coat sterile culture dishes or flasks with Gelatin-Based Coating Solution for 2 min and then aspirate the excess solution before seeding cells. The use of coated-flasks improves cell attachment for certain types of primary cells.Store the solution at 2-8°C.
Lonza Walkersville ReagentPack Subculture Reagents, 100 mL
ReagentPack Subculture Kits have been designed specifically for the passaging of primary cell types. Each kit contains 100 mL each of: Trypsin/EDTA, Trypsin Neutralizing Solution, HEPES Buffered Saline.The ReagentPack Subculture Reagents kit is recommended for use with Lonza's primary cells to ensure effective trypsinization and a successful cell passaging process.Content:1 x Bottle Trypsin/EDTA 100 mL1 x Bottle Trypsin Neutralizing Solution 100 mL1 x Bottle HEPES Buffered Saline Solution 100 mL
STEMCELL Technologies ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator, Size: 2 mL
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens. This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator consists of soluble tetrameric antibody complexes that bind to and cross-link CD3, CD28, and CD2 cell surface ligands, thereby providing the required primary and co-stimulatory signals for T cell activation. This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material (AM) following the framework outlined in USP<1043>. Contact STEMCELL Technologies to learn how we can work with you to qualify this reagent as an AM under an approved investigational new drug (IND) or clinical trial application (CTA).
CELLULAR TECHNOLOGY LTD CTL ANTIAGREGATE WASH 20X 5ML
5 ML. CTL anti-aggregate Washa, c 20x Stabilizes PBMC during thawing and prevents cell loss through aggregation. Cells that die during freeze-thawing release DNa strands that can cause cell clumping and aggregation. Therefore, the use of DNase-containing wash solutions is recommended while thawing PBMC. Moreover, cells show increased metabolic activity after thawing as they compensate for the stress reaction - this needs to be accounted for by using a nutrient-rich medium. CTL anti-aggregate Washa, c Supplement has been specifically formulated for optimal PBMC recovery and functionality after thawing. It contains injection-grade water supplemented with amino acids, vitamins, inorganic salts, and human serum albumin (HSa) in a proprietary composition, saturated and unsaturated fatty acids, and sugars in a ready-to-use formulation, providing a balanced environment for PBMC. For each vial of PBMC to be thawed, 1 vial of CTL anti-aggregate Washa, c Supplement 20x is needed.
Invivogen Hygromycin B Gold, 1g
HygroGold, CAS No.: 31282-04-9, Molecular Formula: C20H37N3O13 HCl, Molecular Weight: 563.5, 1g liquid at 100mg/mL, Pack: 5 x 2mL/TB
Sigma Aldrich Fine Chemicals Biosciences EmbryoMax Advanced KSOM Embryo Medium | 50mL
EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.
Lonza Walkersville HEPES Buffered Saline Solution, 500 mL
HEPES Buffered Saline Solution (BSS), is used to neutralize the complex proteins in growth medium that may inactivate trypsin during trypsinization.HEPES BSS at a fill volume of 100 mL is a component of the ReagentPack Subculture kit (catalog number CC-5024). The 100 mL size is also sold separately (catalog number CC-5022).Content:1 x Bottle HEPES Buffered Solution, 500 mL
Sigma Aldrich Fine Chemicals Biosciences L-Ascorbic acid, 50-81-7, MFCD00064328, 100 mg
Empirical Formula: (Hill Notation) C6H8O6, Molecular Weight: 176.12, powder, cell culture tested, γ-irradiated, Synonym: L-Threoascorbic acid, Antiscorbutic factor, Vitamin C. For use in cell culture applications as an antioxidant and to regenerate tocopherol. L-ascorbic acid has been used: in an osteogenic supplement for the growth of cells, as a component in the differentiation media, and in WS-MSCs (Werner syndrome-mesenchymal stem cells) to study rescuing properties of ascorbic acid on aging defects.
FUJIFILM Biosciences BalanCD CHO Feed 4 - Powder
Maximize productivity inLarge-scale, fed-batch cultures.
Cellular Dynamics International iCell Endothelial Cells Medium Supplement, 50 mL
50 mL Endothelial cells medium supplement, serum-based.
CELLULAR TECHNOLOGY LTD Ctl Anti-Aggregate Wash 20X, Size 1 ml
Ctl Anti-Aggregate Wash 20X, Size 1 Ml. Stabilizes Pbmc During Thawing And Prevents Cell Loss Through Aggregation. Cells That Die During Freeze-Thawing Release Dna Strands That Can Cause Cell Clumping And Aggregation. Therefore, The Use Of Dnase-Containing Wash Solutions Is Recommended While Thawing Pbmc. Moreover, Cells Show Increased Metabolic Activity After Thawing As They Compensate For The Stress Reaction - This Needs To Be Accounted For By Using A Nutrient-Rich Medium. Ctl Anti-Aggregate Wash Supplement Has Been Specifically Formulated For Optimal Pbmc Recovery And Functionality After Thawing. It Contains Injection-Grade Water Supplemented With Amino Acids, Vitamins, Inorganic Salts, And Human Serum Albumin (Hsa) In A Proprietary Composition, Saturated And Unsaturated Fatty Acids, And Sugars In A Ready-To-Use Formulation, Providing A Balanced Environment For Pbmc. For Each Vial Of Pbmc To Be Thawed, 1 Vial Of Ctl Anti-Aggregate Wash Supplement 20X Is Needed.